Assigning and using molecular weights
Posted: Tue Jul 21, 2015 3:07 pm
Hello,
I have been tasked with setting up a SEC for antibody analysis using clarity software and a Agilent HPLC. Having only used Waters/Empower in the past this experience has been something of a learning curve.
I have set up a method running through the GPC module which generates chromatography as expected, I have also ran gel filtration standards through the system but am unable to find how I can assign molecular weights to the peaks within the standard and how this is then used to analyse the molecular weight of my product peaks.
I have no doubt that this is an oversight on my part but having spent two days searching I have no choice but to ask the advice of people who actually know the software.
Thanks in advance.
I have been tasked with setting up a SEC for antibody analysis using clarity software and a Agilent HPLC. Having only used Waters/Empower in the past this experience has been something of a learning curve.
I have set up a method running through the GPC module which generates chromatography as expected, I have also ran gel filtration standards through the system but am unable to find how I can assign molecular weights to the peaks within the standard and how this is then used to analyse the molecular weight of my product peaks.
I have no doubt that this is an oversight on my part but having spent two days searching I have no choice but to ask the advice of people who actually know the software.
Thanks in advance.