Dealing with overintegration because of drift
Posted: Sun Oct 13, 2013 5:02 pm
Not sure if "drift" is the right word. There are two groups of peaks, D and T, at the end of my chromatograms. When they are present, they tend to form a bit of a lump in the baseline. This is not a major problem when there are significant amounts of D (top GC).
However, when there's only a small amount of D present, clarity tends to integrate the whole lump, and D ends up being overintegrated (bottom GC). I'm not sure what setting is best to deal with this. I really just want Clarity to only integrate the ones that are obviously peaks. There is a substantial variation in peak widths across a chromatogram, as the larger peaks in the T group can be 2 or 3 times the size of some other peaks. Any ideas what Integration settings I should use/change?
However, when there's only a small amount of D present, clarity tends to integrate the whole lump, and D ends up being overintegrated (bottom GC). I'm not sure what setting is best to deal with this. I really just want Clarity to only integrate the ones that are obviously peaks. There is a substantial variation in peak widths across a chromatogram, as the larger peaks in the T group can be 2 or 3 times the size of some other peaks. Any ideas what Integration settings I should use/change?