YL HPLC with Agilent 1200 FLD problem
Posted: Tue Oct 30, 2012 4:12 pm
Hello,
I am using a Young-Lin 9100 Series HPLC (9110 Pump, 9131 Thermostat, 9120 UV/Vis det) with an Agilent 1200 series FLD detector (G1321B). Clarity version is 3.0.4.444.
I am encounreing a problem with sottping runs. Analysis time is set into the method as 32 min. The Agilent is set to 27 min run time and 5 post time (I tried 32 min run time, and OFF for post time, and olso OFF for both). After 32 min the sequence says "Stopping", however it does not stop.
I read the Agilent 1100 series HELP file (there is no 1200 series help), and found that LC gradient should have that time ponts - checked, it has. I filled the autosample and other parts analysis times too (thermostat, and others), experimented with the values also. After removing and readding the device in configuration it worked on one machine (with clarity version 4.0.1.700) for a while, but after some configuration the problem reappeared.
I am alsohaving some errors when sending method to device, and of course sometimes the analysis wont start, with "Waiting" in sequence.
I tried the options in the HELP file, and also I am not having a full Agilent 1100 system (for what the HELP file is written).
What other things can I try? I can send you the log files if that helps.
Regards,
Laszlo Lorantfy
I am using a Young-Lin 9100 Series HPLC (9110 Pump, 9131 Thermostat, 9120 UV/Vis det) with an Agilent 1200 series FLD detector (G1321B). Clarity version is 3.0.4.444.
I am encounreing a problem with sottping runs. Analysis time is set into the method as 32 min. The Agilent is set to 27 min run time and 5 post time (I tried 32 min run time, and OFF for post time, and olso OFF for both). After 32 min the sequence says "Stopping", however it does not stop.
I read the Agilent 1100 series HELP file (there is no 1200 series help), and found that LC gradient should have that time ponts - checked, it has. I filled the autosample and other parts analysis times too (thermostat, and others), experimented with the values also. After removing and readding the device in configuration it worked on one machine (with clarity version 4.0.1.700) for a while, but after some configuration the problem reappeared.
I am alsohaving some errors when sending method to device, and of course sometimes the analysis wont start, with "Waiting" in sequence.
I tried the options in the HELP file, and also I am not having a full Agilent 1100 system (for what the HELP file is written).
What other things can I try? I can send you the log files if that helps.
Regards,
Laszlo Lorantfy